从江香猪FoxO 4基因真核表达载体的构建及其在皮下脂肪前体细胞中的表达

doi:10.11843/j.issn.0366-6964.2017.12.006

Abstract

Abstract:

The aim of this study was to clone the coding sequence of FoxO 4 gene of Congjiang Xiang pig and preliminarily explore the function of FoxO 4 gene. The relative expression level of FoxO 4 gene was detected by qRT-PCR in different tissues of Congjiang Xiang pig at the age of 6 month.The recombinant plasmid pEGFP-N3-FoxO4 was constructed by inserting the FoxO 4 gene coding sequence into pEGFP-N3, and then transiently transfected into the subcutaneous preadipocytes of Congjiang Xiang pig by liposome-mediated transfection.The expression levels of FoxO 4, PPARγ, LPL, FAS, ACC, ATGL, HSL in transfected cells were detected by qRT-PCR. The results showed that FoxO 4 gene had the highest expression level in adipose tissue. The constructed plasmid pEGFP-N3-FoxO4 was successfully expressed in subcutaneous preadipocytes. The expression level of LPL, FAS, ACC, ATGL and HSL genes were significantly up-regulated in the subcutaneous preadipocytes when compared to the control group.These results revealed that FoxO 4 gene had certain regulation on fat deposition and might be a key candidate gene for the pork quality, which will provide theoretical basis for further studying the mechanism of fat deposition.

CLC Number:

S828.2

SUN Cheng-juan, XU Hou-qiang, DUAN Zhi-qiang, CHEN Wei, ZHAO Jia-fu, ZHOU Di. Construction of Recombinant Eukaryotic Expression Vector of FoxO 4 Gene from Congjiang Xiang Pig and Its Expression in Subcutaneous Preadipocytes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(12): 2268-2276.

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Construction of Recombinant Eukaryotic Expression Vector of FoxO 4 Gene from Congjiang Xiang Pig and Its Expression in Subcutaneous Preadipocytes

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